MaeN

• Description: Na+/malate symporter
  
  

Gene name	maeN
Synonyms	yufR
Essential	no
Product	Na+/malate symporter
Function	malate uptake
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	47 kDa, 8.411
Gene length, protein length	1344 bp, 448 aa
Immediate neighbours	nupQ, yufS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

• Locus tag: BSU31580

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family

• Paralogous protein(s): CimH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O05256

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: maeN PubMed

• Sigma factor:

• Regulation: induced by malate (MalR)

• Regulatory mechanism: MalR: transcription activation

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:
  • 1A642 (maeN::Tn917, erm) (available from the BGSC or in Jörg Stülke's lab)

• Expression vector:
  • expression of native maeN in B. subtilis: pGP1951 (in pBQ200), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Iwona Sobczak, Juke S Lolkema
The 2-hydroxycarboxylate transporter family: physiology, structure, and mechanism.
Microbiol Mol Biol Rev: 2005, 69(4);665-95
[PubMed:16339740] [WorldCat.org] [DOI] (P p)

Original publications

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947