YwlE

• Description: protein tyrosine phosphatase, protein arginine phosphatase
  
  

Gene name	ywlE
Synonyms	ipc-31d
Essential	no
Product	protein tyrosine phosphatase, protein arginine phosphatase
Function	dephosphorylation of McsB
MW, pI	16 kDa, 6.681
Gene length, protein length	450 bp, 150 aa
Immediate neighbours	ywlF, ywlD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein modification

The gene

Basic information

• Locus tag: BSU36930

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate (according to Swiss-Prot)

• Protein family: low molecular weight phosphotyrosine protein phosphatase family (according to Swiss-Prot)

• Paralogous protein(s): YfkJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • McsB-YwlE (dephosphorylation of McsB) PubMed

• Localization: weak oligomerization, results in inactivation of YwlE PubMed

Database entries

• Structure: 1ZGG

• UniProt: P39155

• KEGG entry: [2]

• E.C. number: 3.1.3.48

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: ywlE::aphA3 availbale from the Gerth lab

ywlE::tet availbale from the Gerth lab ywlE::spec availbale from the Gerth lab

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Gerth lab