RhgR

• Description: transcriptional activator (AraC family) of the rhamnogalacturonan operon
  
  

Gene name	yesS
Synonyms
Essential	no
Product	transcriptional activator of the rhamnogalacturonan operon
Function	regulation of pectin utilization
MW, pI	87 kDa, 7.77
Gene length, protein length	2283 bp, 761 aa
Immediate neighbours	yesR, yesT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, membrane proteins

This gene is a member of the following regulons

YesS regulon

The YesS regulon:

• yesS-yesT-yesU-yesV-yesW-yesX-yesY-yesZ

The gene

Basic information

• Locus tag: BSU07010

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: HPr-binding domain: aa 406 - 510 PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity: interaction with HPr(His)-P (occurs in the absence of preferred carbon sources) stimulates YesS activity PubMed

• Interactions:
  • YesS is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of interactions PubMed
  • HPr(His)-P-YesS PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31522

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yesS-yesT-yesU-yesV-yesW-yesX-yesY-yesZ PubMed

• Sigma factor:

• Regulation: induced by pectin (YesS) PubMed

• Regulatory mechanism: YesS: transcriptional activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Elodie Marchadier, Rut Carballido-López, Sophie Brinster, Céline Fabret, Peggy Mervelet, Philippe Bessières, Marie-Françoise Noirot-Gros, Vincent Fromion, Philippe Noirot
An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach.
Proteomics: 2011, 11(15);2981-91
[PubMed:21630458] [WorldCat.org] [DOI] (I p)

Sandrine Poncet, Maryline Soret, Peggy Mervelet, Josef Deutscher, Philippe Noirot
Transcriptional activator YesS is stimulated by histidine-phosphorylated HPr of the Bacillus subtilis phosphotransferase system.
J Biol Chem: 2009, 284(41);28188-28197
[PubMed:19651770] [WorldCat.org] [DOI] (I p)

Akihito Ochiai, Takafumi Itoh, Akiko Kawamata, Wataru Hashimoto, Kousaku Murata
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl Environ Microbiol: 2007, 73(12);3803-13
[PubMed:17449691] [WorldCat.org] [DOI] (P p)