GlcK

• Description: glucose kinase (D-glucose:ATP)
  
  

Gene name	glcK
Synonyms	yqgR
Essential	no
Product	glucose kinase (D-glucose:ATP)
Function	phosphorylation of the free glucose moiety resulting from cleavage of di-and oligosaccharides
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism
MW, pI	33 kDa, 5.988
Gene length, protein length	963 bp, 321 aa
Immediate neighbours	yqgS, yqgQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources, membrane proteins

The gene

Basic information

• Locus tag: BSU24850

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-glucose = ADP + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: ROK (nagC/xylR) family (according to Swiss-Prot)

• Paralogous protein(s): XylR

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 2QM1 (from Enterococcus faecalis, 38% identity, 52% similarity)

• UniProt: P54495

• KEGG entry: [2]

• E.C. number: 2.7.1.2

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP2 (spc), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stefan Schönert, Sabine Seitz, Holger Krafft, Eva-Anne Feuerbaum, Iris Andernach, Gabriele Witz, Michael K Dahl
Maltose and maltodextrin utilization by Bacillus subtilis.
J Bacteriol: 2006, 188(11);3911-22
[PubMed:16707683] [WorldCat.org] [DOI] (P p)

Lili R Mesak, Felix M Mesak, Michael K Dahl
Expression of a novel gene, gluP, is essential for normal Bacillus subtilis cell division and contributes to glucose export.
BMC Microbiol: 2004, 4;13
[PubMed:15050034] [WorldCat.org] [DOI] (I e)

Lili R Mesak, Felix M Mesak, Michael K Dahl
Bacillus subtilis GlcK activity requires cysteines within a motif that discriminates microbial glucokinases into two lineages.
BMC Microbiol: 2004, 4;6
[PubMed:15018644] [WorldCat.org] [DOI] (I e)

P Skarlatos, M K Dahl
The glucose kinase of Bacillus subtilis.
J Bacteriol: 1998, 180(12);3222-6
[PubMed:9620975] [WorldCat.org] [DOI] (P p)

F Titgemeyer, J Reizer, A Reizer, M H Saier
Evolutionary relationships between sugar kinases and transcriptional repressors in bacteria.
Microbiology (Reading): 1994, 140 ( Pt 9);2349-54
[PubMed:7952186] [WorldCat.org] [DOI] (P p)