Pel

• Description: pectate lyase C
  
  

Gene name	pel
Synonyms
Essential	no
Product	pectate lyase C
Function	degradation of polygalacturonic acid
MW, pI	45 kDa, 8.421
Gene length, protein length	1260 bp, 420 aa
Immediate neighbours	yflT, yflS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU07560

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of specific carbon sources

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to Swiss-Prot)

• Protein family: polysaccharide lyase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide), major constituent of the secretome PubMed

Database entries

• Structure: 2O1D (bound to trisaccharide), 1BN8

• UniProt: P39116

• KEGG entry: [3]

• E.C. number: 4.2.2.2

Expression and regulation

• Operon: pel PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (3.8-fold) (CcpA) PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • CcpA: transcription repression
  • TnrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)