KatX

• Description: catalase, general stress protein
  
  

Gene name	katX
Synonyms	yxlI
Essential	no
Product	catalase (major catalase in spores)
Function	detoxification (degradation) of hydrogen peroxide
MW, pI	62 kDa, 5.341
Gene length, protein length	1641 bp, 547 aa
Immediate neighbours	aag, yxlH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38630

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

sporulation proteins, general stress proteins (controlled by SigB), resistance against oxidative and electrophile stress

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 H₂O₂ = O₂ + 2 H₂O (according to Swiss-Prot)

• Protein family: catalase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94377

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: katX PubMed

• Sigma factor: SigB PubMed, SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF, RsfA) PubMed
  • induced by stress (SigB) PubMed

• Regulatory mechanism:
  • RsfA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Identification and characterization of a new prespore-specific regulatory gene, rsfA, of Bacillus subtilis.
J Bacteriol: 2000, 182(2);418-24
[PubMed:10629188] [WorldCat.org] [DOI] (P p)

A Petersohn, S Engelmann, P Setlow, M Hecker
The katX gene of Bacillus subtilis is under dual control of sigmaB and sigmaF.
Mol Gen Genet: 1999, 262(1);173-9
[PubMed:10503549] [WorldCat.org] [DOI] (P p)

I Bagyan, L Casillas-Martinez, P Setlow
The katX gene, which codes for the catalase in spores of Bacillus subtilis, is a forespore-specific gene controlled by sigmaF, and KatX is essential for hydrogen peroxide resistance of the germinating spore.
J Bacteriol: 1998, 180(8);2057-62
[PubMed:9555886] [WorldCat.org] [DOI] (P p)

L Casillas-Martinez, P Setlow
Alkyl hydroperoxide reductase, catalase, MrgA, and superoxide dismutase are not involved in resistance of Bacillus subtilis spores to heat or oxidizing agents.
J Bacteriol: 1997, 179(23);7420-5
[PubMed:9393707] [WorldCat.org] [DOI] (P p)