Difference between revisions of "Xsa"

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(Xsa moved to Abf2)
 
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#REDIRECT [[Abf2]]
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* '''Description:''' repressor of the arabinose utilization genes <br/><br/>
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{| align="right" border="1" cellpadding="2"
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|-
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''araR''
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|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''araC, yvbS ''
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|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor (LacI family))
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of arabinose utilization
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(araABDLMNPQ-abfA) and of the araE/araR genes
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|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 6.341 
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|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1152 bp, 384 aa
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[araE]]'', ''[[yvbT]]''
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15402&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:araR_context.gif]]
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<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|}
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__TOC__
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<br/><br/>
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=The gene=
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 +
=== Basic information ===
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* '''Locus tag:''' BSU33970
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===Phenotypes of a mutant ===
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 +
=== Database entries ===
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/araR.html]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11913]
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=== Additional information===
 +
 
 +
 
 +
=The protein=
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 +
=== Basic information/ Evolution ===
 +
 
 +
* '''Catalyzed reaction/ biological activity:'''
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* '''Protein family:'''
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* '''Paralogous protein(s):'''
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=== Genes/ operons controlled by AraR ===
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* '''Activated by AraR:'''
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* ''' Repressed by AraR:''' ''[[araA]]-[[araB]]-[[araD]]-[[araL]]-[[araM]]-[[araN]]-[[araP]]-[[araQ]]-[[abfA]]''
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=== Extended information on the protein ===
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* '''Kinetic information:'''
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* '''Domains:'''
 +
 
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* '''Modification:'''
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* '''Cofactor(s):'''
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* '''Effectors of protein activity:'''
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* '''Interactions:'''
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* '''Localization:''' cytoplasm (according to Swiss-Prot)
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=== Database entries ===
 +
 
 +
* '''Structure:'''
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P96711 P96711]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU33970]
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* '''E.C. number:'''
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 +
=== Additional information===
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=Expression and regulation=
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* '''Operon:'''
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* '''[[Sigma factor]]:'''
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* '''Regulation:'''
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** repressed in the absence of arabinose ([[AraR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
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* '''Regulatory mechanism:'''
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** [[AraR]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14973026 PubMed]
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** [[AraR]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
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* '''Additional information:'''
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 +
=Biological materials =
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* '''Mutant:'''
 +
 
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* '''Expression vector:'''
 +
       
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* '''lacZ fusion:'''
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* '''GFP fusion:'''
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* '''two-hybrid system:'''
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 +
* '''Antibody:'''
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 +
=Labs working on this gene/protein=
 +
 
 +
=Your additional remarks=
 +
 
 +
=References=
 +
 
 +
<pubmed>10417639 9045819, </pubmed>
 +
# Mota LJ, Tavares P, Sá-Nogueira I. (1999) Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis. ''Mol Microbiol.'' '''Aug;33(3):''' 476-89. [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
 +
# Sá-Nogueira I, Mota LJ. (1997) Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene. ''J Bacteriol. '' '''Mar;179(5):''' 1598-608. [http://www.ncbi.nlm.nih.gov/sites/entrez/9045819 PubMed]

Revision as of 12:46, 11 June 2009

  • Description: repressor of the arabinose utilization genes

Gene name araR
Synonyms araC, yvbS
Essential no
Product transcriptional repressor (LacI family))
Function regulation of arabinose utilization

(araABDLMNPQ-abfA) and of the araE/araR genes

MW, pI 43 kDa, 6.341
Gene length, protein length 1152 bp, 384 aa
Immediate neighbours araE, yvbT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AraR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU33970

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Genes/ operons controlled by AraR

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed in the absence of arabinose (AraR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, L J Mota
Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene.
J Bacteriol: 1997, 179(5);1598-608
[PubMed:9045819] [WorldCat.org] [DOI] (P p)

  1. Mota LJ, Tavares P, Sá-Nogueira I. (1999) Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis. Mol Microbiol. Aug;33(3): 476-89. PubMed
  2. Sá-Nogueira I, Mota LJ. (1997) Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene. J Bacteriol. Mar;179(5): 1598-608. PubMed
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