DacF

• Description: penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacF
Synonyms
Essential	no
Product	penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	43 kDa, 9.912
Gene length, protein length	1167 bp, 389 aa
Immediate neighbours	spoIIAA, punA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU23480

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)

• Protein family: peptidase S11 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P38422

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dacF-spoIIAA-spoIIAB-sigF

• Sigma factor: SigF, SigG

• Regulation: repressed by casamino acids PubMed , expressed during sporulation in the prespore Link to a summary

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed