Difference between revisions of "PurR"
| Line 35: | Line 35: | ||
=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU00470 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 80: | Line 80: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37551 P37551] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37551 P37551] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00470] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Revision as of 12:00, 3 June 2009
- Description: transcription repressor of the pur operon
| Gene name | purR |
| Synonyms | yabI |
| Essential | no |
| Product | transcription repressor of the pur operon |
| Function | regulation of purine biosynthesis |
| MW, pI | 31 kDa, 5.771 |
| Gene length, protein length | 855 bp, 285 aa |
| Immediate neighbours | ispE, yabJ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU00470
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Biological activity: transcriptional repressor of purine biosynthetic operons
- Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed
- Localization:
Database entries
- Swiss prot entry: P37551
- KEGG entry: [3]
- E.C. number:
Additional information
requires YabJ for in vivo activity
Expression and regulation
- Regulation: negative autoregulation, induction in the presence of PRPP PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Shuobo Shi, Tao Chen, Zhigang Zhang, Xun Chen, Xueming Zhao
Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.
Metab Eng: 2009, 11(4-5);243-52
[PubMed:19446032]
[WorldCat.org]
[DOI]
(I p)
Aloke Kumar Bera, Jianghai Zhu, Howard Zalkin, Janet L Smith
Functional dissection of the Bacillus subtilis pur operator site.
J Bacteriol: 2003, 185(14);4099-109
[PubMed:12837784]
[WorldCat.org]
[DOI]
(P p)
Sangita C Sinha, Joseph Krahn, Byung Sik Shin, Diana R Tomchick, Howard Zalkin, Janet L Smith
The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation.
J Bacteriol: 2003, 185(14);4087-98
[PubMed:12837783]
[WorldCat.org]
[DOI]
(P p)
M Weng, H Zalkin
Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.
Curr Microbiol: 2000, 41(1);56-9
[PubMed:10919400]
[WorldCat.org]
[DOI]
(P p)
M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212]
[WorldCat.org]
[DOI]
(P p)
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
