CwlD

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	cwlD
Synonyms
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	spore germination
MW, pI	26 kDa, 9.788
Gene length, protein length	711 bp, 237 aa
Immediate neighbours	ybaK, salA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P50864

• KEGG entry: BSU01530

• E.C. number: 3.5.1.28

Expression and regulation

• Operon:
  • ybaK-cwlD PubMed
  • cwlD PubMed

• Sigma factor:
  • ybaK: SigG PubMed
  • cwlD: SigE PubMed

• Regulation: expressed during sporulation (SigG, SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed