YtsJ

• Description: malic enzyme
  
  

Gene name	ytsJ
Synonyms
Essential	no
Product	NAD-dependent malate dehydrogenase
Function	malate utilization
MW, pI	43 kDa, 5.046
Gene length, protein length	1230 bp, 410 aa
Immediate neighbours	accD, dnaE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

Poor growth with malate as single carbon source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate <--> pyruvate

• Protein family: malic enzymes family (according to Swiss-Prot)

• Paralogous protein(s): MleA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed cytoplasm protein PubMed

Database entries

• Structure:

• Swiss prot entry: O34962

• KEGG entry: BSU29220

• E.C. number: 1.1.1.38 0

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP612 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

1. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed# Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
2. Lerondel G, Doan T, Zamboni N, Sauer U, Aymerich S. (2006) YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis. J Bacteriol. 188: 4727-4736. PubMed
3. Doan, T., P. Servant, S. Tojo, H. Yamaguchi, G. Lerondel, K. Yoshida, Y. Fujita, and S. Aymerich. 2003. The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate. Microbiology 149: 2331-2343. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed