Difference between revisions of "Sandbox"

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* '''Description:''' regulator of [[SigI]] activity <br/><br/>
+
* '''Description:''' Spo0A-P phosphatase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''rsgI ''
+
|''spo0E''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykrI''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || anti-sigma factor
+
|style="background:#ABCDEF;" align="center"| '''Product''' || Spo0A-P phosphatase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[SigI]] activity
+
|style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 4.517  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9 kDa, 6.483  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1143 bp, 381 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 255 bp, 85 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sigI]]'', ''[[sspD]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykvA]]'', ''[[eag]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13219&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13237&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:ykrI_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:spo0E_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
−
 
−
suppresses the growth defect of a ''[[mbl]]'' mutation [http://www.ncbi.nlm.nih.gov/sites/entrez/19114499 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sigI-rsgI.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0E.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13272]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10769]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 52:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' anti-sigma factor for [[SigI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
+
* '''Catalyzed reaction/ biological activity:'''  
  
 
* '''Protein family:'''
 
* '''Protein family:'''
  
−
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[YnzD]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:''' [[RsgI]]-[[SigI]], [[RsgI]]-[[SigI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
+
* '''Interactions:''' [[Spo0A]]-[[Spo0E]],  
  
−
* '''Localization:''' membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU13460]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU13640]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 90: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''[[sigI]]-[[rsgI]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
+
* '''Operon:'''  
  
−
* '''Sigma factor:''' [[SigI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
+
* '''[[Sigma factor]]:'''  
  
−
* '''Regulation:''' induced by heat [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
+
* '''Regulation:'''  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''
+
* '''Additional information:''' Spo0E is degraded by [[FtsH]]
  
 
=Biological materials =
 
=Biological materials =
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Tony Wilkinson]], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
−
# Asai K, Ootsuji T, Obata K, Matsumoto T, Fujita Y, Sadaie Y. (2007) Regulatory role of RsgI in ''sigI'' expression in ''Bacillus subtilis''. ''Microbiology'' '''153:''' 92-101. [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
 
−
# Schirner K, Errington J. (2009) Cell wall regulator {sigma}I specifically suppresses the lethal phenotype of mbl mutants in ''Bacillus subtilis''. ''J. Bacteriol.'' '''191:''' 1404-1413. [http://www.ncbi.nlm.nih.gov/sites/entrez/19114499 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 23:56, 27 April 2009

  • Description: Spo0A-P phosphatase

Gene name spo0E
Synonyms
Essential no
Product Spo0A-P phosphatase
Function initiation of sporulation
MW, pI 9 kDa, 6.483
Gene length, protein length 255 bp, 85 aa
Immediate neighbours ykvA, eag
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Spo0E context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YnzD

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information: Spo0E is degraded by FtsH

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Tony Wilkinson, York University, U.K. homepage

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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