Difference between revisions of "PGP570"
| Line 9: | Line 9: | ||
based on [[pWH844]] | based on [[pWH844]] | ||
| − | in E. coli: ampicillin resistance | + | in ''E. coli'': ampicillin resistance |
| − | similar plasmid: [[pWH844]] | + | similar [[list of plasmids|plasmid]]: [[pWH844]] |
unpublished | unpublished | ||
Revision as of 11:26, 16 April 2009
pGP570
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein, after purification the tag can be cleaved off by thrombin
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
based on pWH844
in E. coli: ampicillin resistance
unpublished
