Difference between revisions of "PAC6"
| Line 9: | Line 9: | ||
constructed in the Rapoport lab | constructed in the Rapoport lab | ||
| − | in E. coli: ampicillin resistance | + | in ''E. coli'': ampicillin resistance |
| − | in B. subtilis: chloramphenocol resistance | + | in ''B. subtilis'': chloramphenocol resistance |
similar vectors: [[pAC5]], [[pAC7]] | similar vectors: [[pAC5]], [[pAC7]] | ||
Revision as of 11:22, 16 April 2009
pAC6
vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: chloramphenocol resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT Mol Microbiol. 25: 65-78. PubMed
