Difference between revisions of "Sandbox"

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* '''Description:''' D-sorbitol dehydrogenase <br/><br/>
+
* '''Description:''' transcription activator of the ''[[gutB]]-[[gutP]]'' operon <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''gutB''
+
|''gutR''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || D-sorbitol dehydrogenase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || transcription activator
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || glucitol utilization  
+
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of glucitol utilization
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 38 kDa, 5.213  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 94 kDa, 6.113  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1059 bp, 353 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2487 bp, 829 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gutR]]'', ''[[gutP]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ydjC]]'', ''[[gutB]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:gutB_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:gutR_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gutBP.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gutR.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10177]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10178]
  
 
=== Additional information===
 
=== Additional information===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU06150]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU06140]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[gutP]]-[[gutB]]''
+
* '''Operon:'''  
  
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
# Ng K, Ye R, Wu XC, Wong SL. (1992) Sorbitol dehydrogenase from Bacillus subtilis. Purification, characterization, and gene cloning. ''J Biol Chem. '' '''Dec 15;267(35):''' 24989-94. [http://www.ncbi.nlm.nih.gov/sites/entrez/1460002 PubMed]
+
# Ye R, Rehemtulla SN, Wong SL. (1994) Glucitol induction in Bacillus subtilis is mediated by a regulatory factor, GutR. ''J Bacteriol.'' '''Jun;176(11):''' 3321-7. [http://www.ncbi.nlm.nih.gov/sites/entrez/8195087 PubMed]
 +
# Ye R, Wong SL. (1994) Transcriptional regulation of the Bacillus subtilis glucitol dehydrogenase gene. ''J Bacteriol.'' '''Jun;176(11):''' 3314-20. [http://www.ncbi.nlm.nih.gov/sites/entrez/8195086 PubMed]
 +
# Poon KK, Chen CL, Wong SL. (2001) Roles of glucitol in the GutR-mediated transcription activation process in Bacillus subtilis: tight binding of GutR to tis binding site. ''J Biol Chem. '' '''Mar 30;276(13):''' 9620-5 [http://www.ncbi.nlm.nih.gov/sites/entrez/11118449 PubMed]

Revision as of 21:02, 15 April 2009

  • Description: transcription activator of the gutB-gutP operon

Gene name gutR
Synonyms
Essential no
Product transcription activator
Function regulation of glucitol utilization
MW, pI 94 kDa, 6.113
Gene length, protein length 2487 bp, 829 aa
Immediate neighbours ydjC, gutB
Hier soll was neues rein
Genetic context
GutR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Ye R, Rehemtulla SN, Wong SL. (1994) Glucitol induction in Bacillus subtilis is mediated by a regulatory factor, GutR. J Bacteriol. Jun;176(11): 3321-7. PubMed
  2. Ye R, Wong SL. (1994) Transcriptional regulation of the Bacillus subtilis glucitol dehydrogenase gene. J Bacteriol. Jun;176(11): 3314-20. PubMed
  3. Poon KK, Chen CL, Wong SL. (2001) Roles of glucitol in the GutR-mediated transcription activation process in Bacillus subtilis: tight binding of GutR to tis binding site. J Biol Chem. Mar 30;276(13): 9620-5 PubMed