Difference between revisions of "Sandbox"

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−
* '''Description:''' small acid-soluble spore protein (minor alpha/beta-type SASP) <br/><br/>
+
* '''Description:''' regulator of [[SigI]] activity <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''sspD''
+
|''rsgI ''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykrI''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || small acid-soluble spore protein (minor alpha/beta-type SASP)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || anti-sigma factor
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[SigI]] activity
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 8.945  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 4.517  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 192 bp, 64 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1143 bp, 381 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rsgI]]'', ''[[ykrK]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sigI]]'', ''[[sspD]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:sspD_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ykrI_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 38: Line 38:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
suppresses the growth defect of a ''[[mbl]]'' mutation [http://www.ncbi.nlm.nih.gov/sites/entrez/19114499 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sspD.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sigI-rsgI.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10788]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13272]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' anti-sigma factor for [[SigI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
  
 
* '''Protein family:'''
 
* '''Protein family:'''
Line 70: Line 72:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:'''
+
* '''Interactions:''' [[RsgI]]-[[SigI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
  
−
* '''Localization:'''
+
* '''Localization:''' membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
Line 80: Line 82:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU13470]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU13460]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 88: Line 90:
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''[[sigI]]-[[rsgI]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
  
−
* '''[[Sigma factor]]:'''  
+
* '''Sigma factor:''' [[SigI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
  
−
* '''Regulation:'''  
+
* '''Regulation:''' induced by heat [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 118: Line 120:
 
=References=
 
=References=
  
 +
# Asai K, Ootsuji T, Obata K, Matsumoto T, Fujita Y, Sadaie Y. (2007) Regulatory role of RsgI in ''sigI'' expression in ''Bacillus subtilis''. ''Microbiology'' '''153:''' 92-101. [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed]
 +
# Schirner K, Errington J. (2009) Cell wall regulator {sigma}I specifically suppresses the lethal phenotype of mbl mutants in ''Bacillus subtilis''. ''J. Bacteriol.'' '''191:''' 1404-1413. [http://www.ncbi.nlm.nih.gov/sites/entrez/19114499 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 20:10, 15 April 2009

  • Description: regulator of SigI activity

Gene name rsgI
Synonyms ykrI
Essential no
Product anti-sigma factor
Function control of SigI activity
MW, pI 43 kDa, 4.517
Gene length, protein length 1143 bp, 381 aa
Immediate neighbours sigI, sspD
Hier soll was neues rein
Genetic context
YkrI context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

suppresses the growth defect of a mbl mutation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: anti-sigma factor for SigI PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: membrane PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by heat PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Asai K, Ootsuji T, Obata K, Matsumoto T, Fujita Y, Sadaie Y. (2007) Regulatory role of RsgI in sigI expression in Bacillus subtilis. Microbiology 153: 92-101. PubMed
  2. Schirner K, Errington J. (2009) Cell wall regulator {sigma}I specifically suppresses the lethal phenotype of mbl mutants in Bacillus subtilis. J. Bacteriol. 191: 1404-1413. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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