Difference between revisions of "Sandbox"

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* '''Description:''' phosphotransacetylase <br/><br/>
+
* '''Description:''' transcriptional activator of the cysJ-cysI operon <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pta''
+
|''cysL''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-88d, ywfJ ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywfK, ipa-89d''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransacetylase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator (LysR family)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || overflow metabolism
+
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of cysteine biosynthesis
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 4.649  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 6.963  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 969 bp, 323 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 897 bp, 299 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cysL]]'', ''[[ywfI]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywfL]]'', ''[[pta]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:pta_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ywfK_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pta.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywfK.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10634]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10635]
  
 
=== Additional information===
 
=== Additional information===
Line 64: Line 64:
 
* '''Domains:'''  
 
* '''Domains:'''  
  
* '''Modification:''' phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
+
* '''Modification:'''
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU37660]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU37650]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 92: Line 92:
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' expression activated by glucose (2.6 fold) (CcpA) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] 
+
* '''Regulation:'''  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
Line 118: Line 118:
 
=References=
 
=References=
  
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149 [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
 
# Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model  bacterium ''Bacillus subtilis''. Mol. Cell. Proteomics 6: 697-707  [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed]
 
# Presecan-Siedel E, Galinier A, Longin R, Deutscher J, Danchin A, Glaser P, Martin-Verstraete I (1999) The catabolite regulation of the pta gene as part of carbon flow pathways in ''Bacillus subtilis''. J Bacteriol 181:6889-6897. [http://www.ncbi.nlm.nih.gov/sites/entrez/10559153 PubMed]
 
# Shin BS, Choi SK, Park SH. 1999. Regulation of the ''Bacillus subtilis'' phosphotransacetylase gene. J. Biochem. 126:333-339. [http://www.ncbi.nlm.nih.gov/sites/entrez/10423526 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 17:14, 15 April 2009

  • Description: transcriptional activator of the cysJ-cysI operon

Gene name cysL
Synonyms ywfK, ipa-89d
Essential no
Product transcriptional regulator (LysR family)
Function regulation of cysteine biosynthesis
MW, pI 34 kDa, 6.963
Gene length, protein length 897 bp, 299 aa
Immediate neighbours ywfL, pta
Hier soll was neues rein
Genetic context
YwfK context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed