Difference between revisions of "Sandbox"

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* '''Description:''' membrane-associated nuclease, catalyzes DNA cleavage during transformation <br/><br/>
+
* '''Description:''' synthesis of spore killing factor <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''nucA''
+
|''skfB''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''comI ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ybcP, ybcQ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || membrane-associated nuclease
+
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || genetic transformation, DNA uptake
+
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of spore killing factor
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 4.677  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 4.934  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 441 bp, 147 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1230 bp, 410 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nin]]'', ''[[tlpC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[skfA]]'', ''[[skfC]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:nucA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ybcP_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/nucA-nin.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/skf.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10838]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12714]
  
 
=== Additional information===
 
=== Additional information===
Line 72: Line 72:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' integral membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/11359569 PubMed]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU03430]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU01920]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[nucA]]-[[nin]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11359569 PubMed]
+
* '''Operon:'''  
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  

Revision as of 15:52, 15 April 2009

  • Description: synthesis of spore killing factor

Gene name skfB
Synonyms ybcP, ybcQ
Essential no
Product unknown
Function synthesis of spore killing factor
MW, pI 45 kDa, 4.934
Gene length, protein length 1230 bp, 410 aa
Immediate neighbours skfA, skfC
Hier soll was neues rein
Genetic context
YbcP context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed