PonA

• Description: penicillin-binding proteins 1A/1B
  
  

Gene name	ponA
Synonyms
Essential	no
Product	penicillin-binding proteins 1A/1B
Function	bifunctional glucosyltransferase/ transpeptidase
MW, pI	99 kDa, 4.752
Gene length, protein length	2742 bp, 914 aa
Immediate neighbours	recU, ypoC
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a mreB mutation PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane associated PubMed , localizes to the spore septum during sporulation Link to a summary

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: constitutive during vegetative growth Link to a summary

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage

References

1. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
2. Kawai, Y., R. A. Daniel, J. Errington (2009) Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix. Mol. Microbiol. 71: 1131-1144. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed