Difference between revisions of "Sandbox"

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* '''Description:''' fructose-specific phosphotransferase system, EIIC component <br/><br/>
+
* '''Description:''' fructose-specific phosphotransferase system, EIID component <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''levF''
+
|''levG''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sacL ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sacL ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || fructose-specific phosphotransferase system, <br/>EIIC component
+
|style="background:#ABCDEF;" align="center"| '''Product''' || fructose-specific phosphotransferase system, EIID component
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || fructose uptake and phosphorylation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || fructose uptake and phosphorylation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 4.395  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 9.935  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 807 bp, 269 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 825 bp, 275 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[levG]]'', ''[[levE]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacC]]'', ''[[levF]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levF_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levG_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levF_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levG_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:levF_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:levG_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
<br/><br/><br/>
+
<br/><br/>
  
 
=The gene=
 
=The gene=
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* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10318]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10319]
  
 
=== Additional information===
 
=== Additional information===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27050]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27040]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 11:58, 6 April 2009

  • Description: fructose-specific phosphotransferase system, EIID component

Gene name levG
Synonyms sacL
Essential no
Product fructose-specific phosphotransferase system, EIID component
Function fructose uptake and phosphorylation
MW, pI 29 kDa, 9.935
Gene length, protein length 825 bp, 275 aa
Immediate neighbours sacC, levF
Gene sequence (+200bp) Protein sequence
Genetic context
LevG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: PTS permease, mannose permease (Man) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose
  • Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
  1. Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed