Difference between revisions of "Sandbox"

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* '''Description:''' galacturonate transport in via proton symport, glucuronate transport in via proton symport <br/><br/>
+
* '''Description:''' repression of the hexuronate utilization operon (uxaC-yjmBCD-uxuA-yjmf-exuTR-uxaBA) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''exuT''
+
|''exuR''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjmG ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjmH ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || hexuronate transporter
+
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor (LacI family)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || hexuronate uptake
+
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of hexuronate utilization
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 9.614  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.846  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1266 bp, 422 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 999 bp, 333 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjmF]]'', ''[[exuR]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[exuT]]'', ''[[uxaB]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/exuT_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/exuR_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/exuT_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/exuR_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:exuT_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:exuR_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/uxaC-yjmBCD-uxuA-yjmF-exuTR-uxaBA.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13210]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13211]
  
 
=== Additional information===
 
=== Additional information===
Line 81: Line 81:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU12360]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU12370]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 00:49, 3 April 2009

  • Description: repression of the hexuronate utilization operon (uxaC-yjmBCD-uxuA-yjmf-exuTR-uxaBA)

Gene name exuR
Synonyms yjmH
Essential no
Product transcriptional repressor (LacI family)
Function regulation of hexuronate utilization
MW, pI 37 kDa, 5.846
Gene length, protein length 999 bp, 333 aa
Immediate neighbours exuT, uxaB
Gene sequence (+200bp) Protein sequence
Genetic context
ExuR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation: repressed by glucose (CcpA)
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed