Difference between revisions of "Sandbox"

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* '''D s r      ''' a          y r    as  E            (T  -         a  a s      ) < r >< r >
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* '''Description:''' acetoin dehydrogenase E1 component (TPP-dependent alpha subunit) <br/><br/>
  
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{| a    ="r    "   r  r=" "     a    =" "  
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{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
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|s y  =" a k r    #ABCDEF;" a    ="     r"|'''G    a  '''
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''a  A''
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|''acoA''
 
|-
 
|-
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|s y  =" a k r    #ABCDEF;" a    ="     r"| '''Sy  y s''' || ''yfjK ''
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yfjK ''
 
|-
 
|-
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|s y  =" a k r    #ABCDEF;" a    ="     r"| '''Ess    a ''' ||  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
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|s y  =" a k r    #ABCDEF;" a    ="     r"| ''' r    ''' || a          y r    as  E            (T  -         a  a s      )
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|style="background:#ABCDEF;" align="center"| '''Product''' || acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
 
|-
 
|-
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|s y  =" a k r    #ABCDEF;" a    ="     r"|'''F      ''' || a            za   
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|style="background:#ABCDEF;" align="center"|'''Function''' || acetoin utilization
 
|-
 
|-
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|s y  =" a k r    #ABCDEF;" a    ="     r"| ''' W, I''' ||   kDa, 4 9   
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 4.912 
 
|-
 
|-
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|s y  =" a k r    #ABCDEF;" a    ="     r"| '''G          , r            ''' || 999   ,     aa  
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 999 bp, 333 aa  
 
|-
 
|-
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|s y  =" a k r    #ABCDEF;" a    ="     r"|'''I    a          rs''' || ''[[yfjL]]'', ''[[a  B]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfjL]]'', ''[[acoB]]''
 
|-
 
|-
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|s y  =" a k r    #FAF CC;" a    ="     r"|'''[       s      k     -              a  A_            x     G    s q          (+     )  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/acoA_nucleotide.txt     Gene sequence     (+200bp)  ]'''  
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|s y  =" a k r    #FAF CC;" a    ="     r"|'''[       s      k    -             a  A_ r      x  r      s q    ]'''
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/acoA_protein.txt Protein sequence]'''
 
|-
 
|-
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|   s a =" " | '''G            x ''' < r > [[I a  a  A_    x    f]]
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|colspan="2" | '''Genetic context''' <br/> [[Image:acoA_context.gif]]
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  <   a    ="r    "> <s a  >T  s  a    as k    y  r        y [             s  as  r fr S    L s  S    L s ]< s a  ><   >
+
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
 
|}
 
|}
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__TOC__
 
__TOC__
  
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< r >< r >
+
<br/><br/>
  
−
=T      =
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=The gene=
  
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=== Bas    f r a    ===
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=== Basic information ===
  
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* '''C  r  a  s '''
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* '''Coordinates:'''
  
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===     y  s  f a   a  ===
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===Phenotypes of a mutant ===
  
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=== Da a as    r  s ===
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=== Database entries ===
  
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* '''DBTBS   ry ''' [           s    j  COG r  a  ABCL    ]
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acoABCL.html]
  
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* '''S    L s    ry ''' [             s  as  r fr S    L s            ?   _  a  +BG    ]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12558]
  
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=== A      a    f r a    ===
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=== Additional information===
  
  
−
=T    r    =
+
=The protein=
  
−
=== Bas    f r a      E        ===
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=== Basic information/ Evolution ===
  
−
* '''Ca a yz  r a              a  a      y '''  
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* '''Catalyzed reaction/ biological activity:'''  
  
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* ''' r      fa  y '''
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* '''Protein family:'''
  
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* ''' ara    s  r    (s) '''
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* '''Paralogous protein(s):'''
  
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=== Ex        f r a            r      ===
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=== Extended information on the protein ===
  
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* '''K        f r a    '''
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* '''Kinetic information:'''
  
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* '''D  a  s '''  
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* '''Domains:'''  
  
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* '''   f  a    '''
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* '''Modification:'''
  
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* '''C fa  r(s) '''
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* '''Cofactor(s):'''
  
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* '''Eff    rs  f  r      a      y '''
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* '''Effectors of protein activity:'''
  
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* '''I  ra    s '''
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* '''Interactions:'''
  
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* '''L  a  za    ''' Cy    as  (H        s) [                           s  s    r z  6479  7      ]
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* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
  
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=== Da a as    r  s ===
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=== Database entries ===
  
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* '''S r    r  '''
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* '''Structure:'''
  
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* '''S  ss  r      ry '''
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* '''Swiss prot entry:'''
  
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* '''KEGG   ry ''' [                 j      -       _    ? s +BSU  6 ]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU08060]
  
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* '''E C       r '''
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* '''E.C. number:'''
  
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=== A      a    f r a    ===
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=== Additional information===
  
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=Ex r ss    a  r    a    =
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=Expression and regulation=
  
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* '''O  r  ''' ''[[a  A]]-[[a  B]]-[[a  C]]-[[a  L]]'' [                           s  s    r z    74 9        ]
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* '''Operon:''' ''[[acoA]]-[[acoB]]-[[acoC]]-[[acoL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
  
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* '''S  a fa  r ''' [[S  L]] [                           s  s    r z    74 9        ]
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* '''Sigma factor:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
  
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* '''R    a    ''' r  r ss    y      s  ([[C  A]]) [                                                  ],           y a      ([[A  R]]) [                           s  s    r z    74 9        ]
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* '''Regulation:''' repressed by glucose ([[CcpA]]) , induced by acetoin ([[AcoR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
  
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* '''R    a  ry    a  s  ''' [[A  R]]   ra s r      a    a    (   ra          [[S  L]]-   a      RNA   y  ras ) [                           s  s    r z    74 9        ]
+
* '''Regulatory mechanism:''' [[AcoR]]: transcription activation (interaction with [[SigL]]-containing RNA polymerase) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
  
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* '''A      a    f r a    '''  
+
* '''Additional information:'''  
  
−
=B      a  a  r a s =
+
=Biological materials =
  
−
* '''   a  '''
+
* '''Mutant:'''
  
−
* '''Ex r ss        r '''
+
* '''Expression vector:'''
 
          
 
          
−
* ''' a Z f s    '''
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* '''lacZ fusion:'''
  
−
* '''GF  f s    '''
+
* '''GFP fusion:'''
  
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* '''   - y r  sys    '''  
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* '''two-hybrid system:'''  
  
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* '''A      y '''
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* '''Antibody:'''
  
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=La s  rk          s      r    =
+
=Labs working on this gene/protein=
  
−
[[       D  ar      ]], as  r I s      , ar s, Fra    [           as  r fr     asys          -     -   r    r      ar      s-s      f q  s    r              s- - r    s      - -       - s- a  r  s- a      s-a- ra - s  f  s-   r s- - - q    H    a  ]
+
[[Michel Debarbouille]], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
  
−
=Y  r a      a  r  arks=
+
=Your additional remarks=
  
−
=R f r    s=
+
=References=
  
−
# B    k    a  (   ) Tra s r      a  r f      f      x r ss      r s  s          s    ''Ba    s s      s'' r    a      f        ra    a      a  ays  ''   a  E  '' ''' '''   - 49 [                                                  ]
+
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149 
−
#         a  (   6) Sys  a      a  sa      f  r    s f s          r    f  r s      r        ''Ba    s s      s''       f  a      f      r    s a      DNA r    a    fa  ry '' r      s'' '''6 '''     - 46  [                           s  s    r z  6479  7      ]
+
# Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in ''Bacillus subtilis'': identification of new proteins at the DNA replication factory ''Proteomics'' '''6:''' 2135-2146. [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
−
# A  , N O , B    , J , Ra    r , G , a  Dé ar      é,   (   ) R    a      f    a        a a      a  ay  s    r      y s  a L   Ba    s s      s  J Ba  r        , 497-   4  [                           s  s    r z    74 9        ]
+
# Ali, N. O., Bignon, J., Rapoport, G., and Débarbouillé, M. (2001) Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis. J. Bacteriol. 183, 2497-2504. [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
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# A    r , A    r  & A    r  (y ar) T    ''J  r a '' '''       ''' a  - a    [                           s  s    r z  ID      ]
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 23:12, 2 April 2009

  • Description: acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)

Gene name acoA
Synonyms yfjK
Essential no
Product acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
Function acetoin utilization
MW, pI 35 kDa, 4.912
Gene length, protein length 999 bp, 333 aa
Immediate neighbours yfjL, acoB
Gene sequence (+200bp) Protein sequence
Genetic context
AcoA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: Cytoplasm (Homogeneous) PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA) , induced by acetoin (AcoR) PubMed
  • Regulatory mechanism: AcoR: transcription activation (interaction with SigL-containing RNA polymerase) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149
  2. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
  3. Ali, N. O., Bignon, J., Rapoport, G., and Débarbouillé, M. (2001) Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis. J. Bacteriol. 183, 2497-2504. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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