SacT

• Description: transcriptional antiterminator for the sacP-sacA operon
  
  

Gene name	sacT
Synonyms	ipa-47d
Essential	no
Product	transcriptional antiterminator
Function	regulation of sucrose utilization
MW, pI	31 kDa, 5.587
Gene length, protein length	828 bp, 276 aa
Immediate neighbours	ywcJ, ywcI
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binding to the mRNA of the sacP-sacA operon, causes transcription antitermination (in presence of sucrose and absence of glucose)

• Protein family: BglG family of antiterminators

• Paralogous protein(s): LicT, SacY, GlcT

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Débarbouillé, M., Arnaud, M., Fouet, A., Klier, A., and Rapoport, G. (1990) The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators. J Bacteriol 172: 3966-3973. PubMed
2. Arnaud, M., Vary, P., Zagorec, M., Klier, A., Débarbouillé, M., Postma, P., and Rapoport, G. (1992) Regulation of the sacPA operon of Bacillus subtilis: identification of phosphotransferase system components involved in SacT activity. J Bacteriol 174: 3161-3170. PubMed
3. Arnaud, M., Débarbouillé, M., Rapoport, G., Saier, M. H., and Reizer, J. (1996) In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis. J Biol Chem 271: 18966-18972. PubMed
4. Aymerich, S. and Steinmetz, M. (1992) Specificity determinants and structural features in the RNA target of the bacterial antiterminator proteins of the BglG/SacY family. Proc. Natl. Acad. Sci. USA 89, 10410-10414. PubMed
5. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed