Difference between revisions of "Sandbox"

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* '''Description:''' two-component sensor kinase that controls degradative enzyme synthesis and genetic competence<br/><br/>
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* '''Description:''' spore coat protein <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''degS''
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|''yeeK''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sacU ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor histidine kinase (EC 2.7.3.-)
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|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || degradative enzyme and competence regulation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 44 kDa, 5.988  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 15 kDa, 6.852  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1155 bp, 385 aa  
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 435 bp, 145 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[degU]]'', ''[[yvyE]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yeeI]]'', ''[[yezE]]''
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/degS_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yeeK_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/degS_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yeeK_protein.txt Protein sequence]'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:degS_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yeeK_context.gif]]
 
|-
 
|-
 
|}
 
|}
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
−
 
+
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/degSU.html]
+
* '''DBTBS entry:''' no entry
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10392]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12825]
  
 
=== Additional information===
 
=== Additional information===
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* '''Domains:'''  
 
* '''Domains:'''  
  
−
* '''Modification:''' phosphorylation on Ser-76 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
+
* '''Modification:'''
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:''' [[DegS]]-[[DegU]]
+
* '''Interactions:'''
  
−
* '''Localization:'''
+
* '''Localization:''' assembles into the spore integument [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 +
 +
[[CotE]], [[SafA]], and [[SpoVID]] are required for proper localization, clolocalizes with [[YabG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
  
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''''' [[degS]]-[[degU]]''
+
* '''Operon:''' ''yeeK'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
  
−
* '''Sigma factor:''' [[SigA]]
+
* '''Sigma factor:''' [[SigK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
  
−
* '''Regulation:''' induced in the presence of ammonium ([[TnrA]])
+
* '''Regulation:''' expression startts 5 hrs after the onset of sporulation, activated by [[GerE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
  
−
* '''Regulatory mechanism:''' [[TnrA]]: repression, [[DegU]]: positive autoregulation
+
* '''Regulatory mechanism:'''  
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
−
 
−
[[Tarek Msadek]], Institut Pasteur, Paris, France
 
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
−
# Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model  bacterium ''Bacillus subtilis''. Mol. Cell. Proteomics 6: 697-707  [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed]
+
# Takamatsu et al. (2009) Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy''J. Bacteriol.'' '''191:''' 1220-1229. [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
−
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 13:07, 16 March 2009

  • Description: spore coat protein

Gene name yeeK
Synonyms
Essential no
Product spore coat protein
Function unknown
MW, pI 15 kDa, 6.852
Gene length, protein length 435 bp, 145 aa
Immediate neighbours yeeI, yezE
Gene sequence (+200bp) Protein sequence
Genetic context
YeeK context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: assembles into the spore integument PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

CotE, SafA, and SpoVID are required for proper localization, clolocalizes with YabG PubMed

Expression and regulation

  • Regulation: expression startts 5 hrs after the onset of sporulation, activated by GerE PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Takamatsu et al. (2009) Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopyJ. Bacteriol. 191: 1220-1229. PubMed
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