Difference between revisions of "Sandbox"

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* '''Description:''' penicillin-binding protein 2C <br/><br/>
+
* '''Description:''' write here <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pbpF''
+
|''pbpD''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ponA ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || penicillin-binding protein 2C
+
|style="background:#ABCDEF;" align="center"| '''Product''' || penicillin-binding protein 4
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || involved in spore peptidoglycan synthesis
+
|style="background:#ABCDEF;" align="center"|'''Function''' ||  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 79 kDa, 7.078  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 70 kDa, 9.307  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2142 bp, 714 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1872 bp, 624 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhgC]]'', ''[[hemE]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuxJ]]'', ''[[yuxK]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/pbpF_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/pbpD_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/pbpF_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/pbpD_protein.txt Protein sequence]'''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
 
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:pbpF_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:pbpD_context.gif]]
 
|-
 
|-
 
|}
 
|}
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=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pbpF.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pbpD-yuxK.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10428]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10977]
  
 
=== Additional information===
 
=== Additional information===
Line 74: Line 74:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Operon:'''  
 
* '''Operon:'''  
  
* '''Sigma factor:''' [[SigA]], [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
+
* '''Sigma factor:'''  
  
* '''Regulation:''' expressed in mother cell and prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
+
* '''Regulation:'''  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab
+
* '''two-hybrid system:'''  
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 16:49, 9 March 2009

  • Description: write here

Gene name pbpD
Synonyms
Essential no
Product penicillin-binding protein 4
Function
MW, pI 70 kDa, 9.307
Gene length, protein length 1872 bp, 624 aa
Immediate neighbours yuxJ, yuxK
Gene sequence (+200bp) Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context
PbpD context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed