Difference between revisions of "AprE"

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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/aprE_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
 
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/aprE_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
 
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/aprE_protein.txt Protein sequence]'''
 
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/aprE_protein.txt Protein sequence]'''
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|-
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|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:aprE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:aprE_context.gif]]

Revision as of 14:02, 23 February 2009

  • Description: write here

Gene name aprE
Synonyms sprE
Essential no
Product serine alkaline protease (subtilisin E) (EC 3.4.21.62)
Function
MW, pI 39 kDa, 9.342
Gene length, protein length 1143 bp, 381 aa
Immediate neighbours yhfN, yhfO
Gene sequence (+200bp) Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context
AprE context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information: the mRNA is extremely stable (more than 25 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Hambraeus, G., Persson, M. & Rutberg, B. (2000). The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis. Microbiology 146, 3051-3059. PubMed
  2. Hambraeus, G., Karhumaa, K. & Rutberg, B. (2002). A 5' stem-loop and ribosome binding but not translation are important for the stability of Bacillus subtilis aprE leader mRNA. Microbiology 148, 1795-1803. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed