RecJ

• Description: single-strand DNA-specific exonuclease
  
  

Gene name	yrvE
Synonyms	recJ
Essential	no
Product	single-strand DNA-specific exonuclease
Function	DNA repair
Gene expression levels in SubtiExpress: yrvE
Interactions involving this protein in SubtInteract: YrvE
Metabolic function and regulation of this protein in SubtiPathways: yrvE
MW, pI	87 kDa, 5.181
Gene length, protein length	2358 bp, 786 aa
Immediate neighbours	apt, yrvD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

stringent response, Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU27620

Database entries

• BsubCyc: BSU27620

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: recJ family (according to Swiss-Prot)

• Paralogous protein(s): YorK

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SsbA-YrvE PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27620

• Structure:

• UniProt: O32044

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yrvE-apt PubMed

• Expression browser: yrvE PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

• Mutant:
  • GP895 (recJ::kan), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)