Difference between revisions of "YabT"
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| − | * '''Description:''' [[protein kinases and phosphatases|Ser/Thr kinase]], controls DNA integrity during spore development <br/><br/> | + | * '''Description:''' [[protein kinases and phosphatases|Ser/Thr kinase]], controls [[translation]] and DNA integrity during spore development <br/><br/> |
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
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** phosphorylation of [[RecA]] on Ser-2 {{PubMed|23634894}} | ** phosphorylation of [[RecA]] on Ser-2 {{PubMed|23634894}} | ||
| + | ** phosphorylation of [[EF-Tu|tufA]] on Thr-63 during [[sporulation]] {{PubMed|26056311}} | ||
* '''Protein family:''' [[protein kinases and phosphatases|protein kinase]] domain (according to Swiss-Prot) | * '''Protein family:''' [[protein kinases and phosphatases|protein kinase]] domain (according to Swiss-Prot) | ||
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** [[YabT]]-[[RecA]] {{PubMed|23634894}} | ** [[YabT]]-[[RecA]] {{PubMed|23634894}} | ||
** [[AbrB]]-[[YabT]] {{PubMed|24731262}} | ** [[AbrB]]-[[YabT]] {{PubMed|24731262}} | ||
| + | ** [[AbrB]]-[[YabT]] {{PubMed|26056311}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| Line 147: | Line 148: | ||
=References= | =References= | ||
| − | <pubmed>16497325,23634894, 24731262 25278935 </pubmed> | + | <pubmed>16497325,23634894, 24731262 25278935 26056311</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:19, 15 June 2015
- Description: Ser/Thr kinase, controls translation and DNA integrity during spore development
| Gene name | yabT |
| Synonyms | |
| Essential | no |
| Product | Ser/Thr kinase |
| Function | control of DNA integrity during spore development |
| Gene expression levels in SubtiExpress: yabT | |
| Interactions involving this protein in SubtInteract: YabT | |
| MW, pI | 37 kDa, 9.943 |
| Gene length, protein length | 1014 bp, 338 aa |
| Immediate neighbours | yabS, tilS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
protein modification, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00660
Phenotypes of a mutant
- increased sensitivity to DNA damage during spore development PubMed
Database entries
- BsubCyc: BSU00660
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- phosphorylation of RecA on Ser-2 PubMed
- phosphorylation of tufA on Thr-63 during sporulation PubMed
- Protein family: protein kinase domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- autophosphorylation is stimulated by non-specific binding to DNA PubMed
- Localization:
- colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed
Database entries
- BsubCyc: BSU00660
- Structure:
- UniProt: P37562
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP577 (erm), available in Jörg Stülke's lab
- Expression vector:
- purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
- purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
- purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
- lacZ fusion:
- translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
