YwbH

• Description: putative holin
  
  

Gene name	ywbH
Synonyms	ipa-23r, cidA
Essential	no
Product	putative holin
Function	unknown
Gene expression levels in SubtiExpress: ywbH
MW, pI	14 kDa, 9.591
Gene length, protein length	384 bp, 128 aa
Immediate neighbours	ywbI, ywbG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, proteins of unknown function

The gene

Basic information

• Locus tag: BSU38320

Phenotypes of a mutant

• • ywbHG mutant is delayed in pellicle formation (Chen et al)
  • ywbHG-yxaKC-ysbAB triple mutant is severely delayed in pellicle formation (Chen et al)
  • ywbHG overexpression strain has more robust pellicle formation (Chen et al)

Database entries

• BsubCyc: BSU38320

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: CidA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU38320

• Structure:

• UniProt: P39591

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ywbH PubMed

• Sigma factor:

• Regulation:
  • ywbHG expression is induced by acetate (Chen et al)

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kelly C Rice, Ethan E Mann, Jennifer L Endres, Elizabeth C Weiss, James E Cassat, Mark S Smeltzer, Kenneth W Bayles
The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus.
Proc Natl Acad Sci U S A: 2007, 104(19);8113-8
[PubMed:17452642] [WorldCat.org] [DOI] (P p)

Kelly C Rice, Jeremy B Nelson, Toni G Patton, Soo-Jin Yang, Kenneth W Bayles
Acetic acid induces expression of the Staphylococcus aureus cidABC and lrgAB murein hydrolase regulator operons.
J Bacteriol: 2005, 187(3);813-21
[PubMed:15659658] [WorldCat.org] [DOI] (P p)