Tmk

• Description: thymidylate kinase
  
  

Gene name	tmk
Synonyms	yaaP
Essential	yes PubMed
Product	thymidylate kinase
Function	TTP biosynthesis
Gene expression levels in SubtiExpress: tmk
Metabolic function and regulation of this protein in SubtiPathways: tmk
MW, pI	23 kDa, 4.866
Gene length, protein length	636 bp, 212 aa
Immediate neighbours	yaaO, darA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

The gene

Basic information

• Locus tag: BSU00280

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU00280

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + dTMP = ADP + dTDP (according to Swiss-Prot)

• Protein family: thymidylate kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU00280

• Structure: 2CCK (from Staphylococcus aureus, 53% identity, 74% similarity) PubMed

• UniProt: P37537

• KEGG entry: [2]

• E.C. number: 2.7.4.9

Expression and regulation

• Operon:

• Expression browser: tmk PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody:

References

Masayo Kotaka, Balvinder Dhaliwal, Jingshan Ren, Charles E Nichols, Richard Angell, Michael Lockyer, Alastair R Hawkins, David K Stammers
Structures of S. aureus thymidylate kinase reveal an atypical active site configuration and an intermediate conformational state upon substrate binding.
Protein Sci: 2006, 15(4);774-84
[PubMed:16522804] [WorldCat.org] [DOI] (P p)