Difference between revisions of "SacT"
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' GP429 (spc), available in [[Stülke]] lab | + | * '''Mutant:''' GP429 (spc), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | ** for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP166, available in [[Stülke]] lab | + | ** for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP166, available in [[Jörg Stülke]]'s lab |
| − | ** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP426, available in [[Stülke]] lab | + | ** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP426, available in [[Jörg Stülke]]'s lab |
| − | ** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP427, available in [[Stülke]] lab | + | ** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP427, available in [[Jörg Stülke]]'s lab |
| − | ** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP439, available in [[Stülke]] lab | + | ** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP439, available in [[Jörg Stülke]]'s lab |
| − | ** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP440, available in [[Stülke]] lab | + | ** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP440, available in [[Jörg Stülke]]'s lab |
| − | ** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP571, available in [[Stülke]] lab | + | ** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP571, available in [[Jörg Stülke]]'s lab |
| − | ** for expression of the RNA-binding domain in ''B. subtilis'', in [[pBQ200]]: pGP446, available in [[Stülke]] lab | + | ** for expression of the RNA-binding domain in ''B. subtilis'', in [[pBQ200]]: pGP446, available in [[Jörg Stülke]]'s lab |
| − | ** for expression, purification of [[sacT]]-full length in ''B. subtilis'' with C-terminal Strep-tag, in [[pGP382]]: pGP1064, available in [[Stülke]] lab | + | ** for expression, purification of [[sacT]]-full length in ''B. subtilis'' with C-terminal Strep-tag, in [[pGP382]]: pGP1064, available in [[Jörg Stülke]]'s lab |
| − | ** for expression, purification of [[sacT]]-full length in ''B. subtilis'' with N-terminal Strep-tag, in [[pGP380]]: pGP1068, available in [[Stülke]] lab | + | ** for expression, purification of [[sacT]]-full length in ''B. subtilis'' with N-terminal Strep-tag, in [[pGP380]]: pGP1068, available in [[Jörg Stülke]]'s lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
| − | * '''GFP fusion:''' GP1227 (spc, based on [[pGP1870]]), available in | + | * '''GFP fusion:''' GP1227 (spc, based on [[pGP1870]]), available in [[Jörg Stülke]]'s lab |
| − | * '''YFP fusion:''' GP1231 (spc, based on [[pGP1871]]), available in | + | * '''YFP fusion:''' GP1231 (spc, based on [[pGP1871]]), available in [[Jörg Stülke]]'s lab |
| − | * '''FLAG-tag construct:''' GP1223 (spc, based on [[pGP1331]]), available in | + | * '''FLAG-tag construct:''' GP1223 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab |
* '''two-hybrid system:''' | * '''two-hybrid system:''' | ||
Revision as of 08:56, 4 March 2015
- Description: transcriptional antiterminator for the sacP-sacA-ywdA operon
| Gene name | sacT |
| Synonyms | ipa-47d |
| Essential | no |
| Product | transcriptional antiterminator |
| Function | regulation of sucrose utilization |
| Gene expression levels in SubtiExpress: sacT | |
| Interactions involving this protein in SubtInteract: SacT | |
| Metabolic function and regulation of this protein in SubtiPathways: sacT | |
| MW, pI | 31 kDa, 5.587 |
| Gene length, protein length | 828 bp, 276 aa |
| Immediate neighbours | ywcJ, ywcI |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
| Expression at a glance PubMed 500px | |
Contents
Categories containing this gene/protein
utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins
This gene is a member of the following regulons
The SacT regulon: sacP-sacA-ywdA
The gene
Basic information
- Locus tag: BSU38070
Phenotypes of a mutant
Database entries
- BsubCyc: BSU38070
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binding to the mRNA of the sacP-sacA operon, causes transcription antitermination (in presence of sucrose and absence of glucose)
- Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU38070
- Structure:
- UniProt: P26212
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- repressed by casamino acids PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP429 (spc), available in Jörg Stülke's lab
- Expression vector:
- for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP166, available in Jörg Stülke's lab
- for expression, purification of PRD-1 in E. coli with N-terminal His-tag, in pWH844: pGP426, available in Jörg Stülke's lab
- for expression, purification of PRD-2 in E. coli with N-terminal His-tag, in pWH844: pGP427, available in Jörg Stülke's lab
- for expression, purification of PRD-1 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP439, available in Jörg Stülke's lab
- for expression, purification of PRD-2 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP440, available in Jörg Stülke's lab
- for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP571, available in Jörg Stülke's lab
- for expression of the RNA-binding domain in B. subtilis, in pBQ200: pGP446, available in Jörg Stülke's lab
- for expression, purification of sacT-full length in B. subtilis with C-terminal Strep-tag, in pGP382: pGP1064, available in Jörg Stülke's lab
- for expression, purification of sacT-full length in B. subtilis with N-terminal Strep-tag, in pGP380: pGP1068, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion: GP1227 (spc, based on pGP1870), available in Jörg Stülke's lab
- YFP fusion: GP1231 (spc, based on pGP1871), available in Jörg Stülke's lab
- FLAG-tag construct: GP1223 (spc, based on pGP1331), available in Jörg Stülke's lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
