Era

• Description: GTP-binding protein, involved in the assembly of the 30S ribosomal subunit
  
  

Gene name	era
Synonyms	bex, yqfH
Essential	yes PubMed
Product	GTP-binding protein
Function	ribosome assembly
Gene expression levels in SubtiExpress: era
MW, pI	33 kDa, 8.376
Gene length, protein length	903 bp, 301 aa
Immediate neighbours	yqzL, cdd
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU25290

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU25290

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP

• Protein family: Era subfamily (according to Swiss-Prot) Era/Obg family

• Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Era-16S rRNA PubMed

• Localization:

Database entries

• BsubCyc: BSU25290

• Structure: 3IEV (Era from Aquifex aeolicus, 50% identity/ 77% similarity, complex with the 3' end of 16S rRNA) PubMed

• UniProt: P42182

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cdd-era (according to DBTBS)

• Expression browser: era PubMed

• Sigma factor: SigA PubMed, SigB PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP837, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP845, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

References

Original publications

Chao Tu, Xiaomei Zhou, Joseph E Tropea, Brian P Austin, David S Waugh, Donald L Court, Xinhua Ji
Structure of ERA in complex with the 3' end of 16S rRNA: implications for ribosome biogenesis.
Proc Natl Acad Sci U S A: 2009, 106(35);14843-8
[PubMed:19706445] [WorldCat.org] [DOI] (I p)

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)

P Z Wang, R H Doi
Overlapping promoters transcribed by bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases.
J Biol Chem: 1984, 259(13);8619-25
[PubMed:6330116] [WorldCat.org] (P p)