BrnQ

• Description: branched-chain amino acid transporter
  
  

Gene name	brnQ
Synonyms	yrdJ
Essential	no
Product	branched-chain amino acid transporter
Function	uptake of branched-chain amino acids
Gene expression levels in SubtiExpress: brnQ
Metabolic function and regulation of this protein in SubtiPathways: brnQ
MW, pI	46 kDa, 9.777
Gene length, protein length	1320 bp, 440 aa
Immediate neighbours	yrdK, azlD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AzlB regulon

The gene

Basic information

• Locus tag: BSU26690

Phenotypes of a mutant

• no phenotype for the single mutant, the triple bcaP brnQ braB mutant is strongly impaired in the transport of isoleucine and valine at low concentrations PubMed

Database entries

• BsubCyc: BSU26690

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • high affinity uptake of isoleucine and valine PubMed

• Protein family: branched chain amino acid transporter family (according to Swiss-Prot)

• Paralogous protein(s): BraB

Extended information on the protein

• Kinetic information:
  • for Ile: Km ≈ 16 μM and Vmax ≈ 25 nmol/min/ mg protein at 37°C PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU26690

• Structure:

• UniProt: P94499

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: azlB-azlC-azlD-brnQ-yrdK PubMed

• Expression browser: brnQ PubMed

• Sigma factor:

• Regulation: repressed by AzlB PubMed

• Regulatory mechanism: AzlB: transcriptional repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris R Belitsky
Role of branched-chain amino acid transport in Bacillus subtilis CodY activity.
J Bacteriol: 2015, 197(8);1330-8
[PubMed:25645558] [WorldCat.org] [DOI] (I p)

B R Belitsky, M C Gustafsson, A L Sonenshein, C Von Wachenfeldt
An lrp-like gene of Bacillus subtilis involved in branched-chain amino acid transport.
J Bacteriol: 1997, 179(17);5448-57
[PubMed:9287000] [WorldCat.org] [DOI] (P p)