fusA

fusA
168

elongation factor G, catalyzes translocation steps of the ribosome after peptide bond formation, facilitates movement of tRNA and mRNA by one codon

Locus
BSU_01120
Molecular weight
76.36 kDa
Isoelectric point
4.61
Protein length
Gene length
Function
translation
Product
elongation factor G
Essential
yes
Synonyms
fusA, fus

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0480 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
130,684 132,762
Phenotypes of a mutant
essential PubMed
specific point mutations affecting FusA facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate PubMed
The protein
Catalyzed reaction/ biological activity
hydrolyses GTP
catalyzes translocation steps of the ribosome after peptide bond formation, facilitates movement of tRNA and mRNA by one codon PubMed
Protein family
tr-type G domain (aa 8-282) (according to UniProt)
Structure
2XEX (PDB) (from Staphylococcus aureus, 78.35% identity) PubMed
1ELO (PDB) (from Thermus thermophilus) PubMed
4BTC (PDB), 4BTD (PDB), the Thermus thermophilus EF-Gribosome complex in a pretranslocation state PubMed
Modification
phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) PubMed, PubMed
phosphorylated on Tyr-339 PubMed
cytoplasm (according to UniProt)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Description
Regulation
Rel dependent downregulation (Class I) during stringent response PubMed
the mRNA is processed between rplGB and rpsL by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Regulatory mechanism
stringent response: negative regulation, PubMed, in stringent response
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rplGB->tufA

2025-02-18 05:37:17

Jstuelk

114

d846304cc2bd15e30cde627ac53b7d142c24fbb5

614BD7852C60161107389373FB106F44BAE4C835

Biological materials
Expression vectors
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP840, available in Jrg Stlke's lab
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP848, available in Jrg Stlke's lab
References
Reviews
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Original Publications
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297844645CCCF40CB40E84339997F6610883DF4E

Page visits: 6222

Time of last update: 2025-02-24 20:47:29

Author of last update: Jstuelk