Difference between revisions of "List of plasmids"
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==Labelling of a protein with a triple FLAG tag== | ==Labelling of a protein with a triple FLAG tag== | ||
| + | * [[pGP1087]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter | ||
* [[pGP1331]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter | * [[pGP1331]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter | ||
| − | * [[ | + | * [[pGP1370]]: replicative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, high expression due to the strong ''[[degQ]]''(Hy) promoter from [[pBQ200]] |
==Expression/ purification in ''B. subtilis''== | ==Expression/ purification in ''B. subtilis''== | ||
Revision as of 14:14, 24 November 2014
Here you can find a list of Bacillus subtilis plasmids:
Contents
- 1 Fusion to reporter genes
- 2 Labelling of a protein with a flourescent tag
- 3 Labelling of a protein with a triple FLAG tag
- 4 Expression/ purification in B. subtilis
- 5 Expression/ purification in E. coli
- 6 Construction of (markerless) deletions
- 7 A collection of biobricks for the work with B. subtilis
Fusion to reporter genes
- pAC5: translational lacZ-fusions (chloramphenicol resistence)
- pAC6: transcriptional lacZ-fusions (cat resistence)
- pAC7: translational lacZ-fusions (kanamycin resistence)
- pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed
- a suite of vectors for ectopic insertion of GFP, CFP and IYFP transcriptional fusions in single copy at the amyE and bglS loci PubMed
- List of GFP variants useful for various fusions (IPTG inducible Phyperspank with amyE integration) PubMed
Labelling of a protein with a flourescent tag
- pBP43: integrative plasmid for the fusion of monomeric GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, this is the preferred plasmid for constructing GFP fusions!
- pGP1871: integrative plasmid for the fusion of YFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter
- pGP1080: integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, allows express of the downstream gene by the P(spac) promoter, ATTENTION: This plasmid encodes the dimeric GFP that tends to produce artifacts!!!
- pGP1870: integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, ATTENTION: This plasmid encodes the dimeric GFP that tends to produce artifacts!!!
- ectopic integration vectors for generating fluorescent promoter fusions in Bacillus subtilis with minimal dark noise PubMed
Labelling of a protein with a triple FLAG tag
- pGP1087: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter
- pGP1331: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
- pGP1370: replicative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, high expression due to the strong degQ(Hy) promoter from pBQ200
Expression/ purification in B. subtilis
- pBP26: expression of YFP in B. subtilis and in E. coli
- pBP27: expression of CFP in B. subtilis and in E. coli
- pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
- pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
- pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
- pGP886: integration vector (integrates in xkdE); allowing the expression of genes under the control of the xylose-inducible PxylA promoter in B. subtilis
- pGP888: integration vector (integrates in ganA); allowing the expression of genes under the control of the xylose-inducible PxylA promoter in B. subtilis
- pGP1459: integration vector (integrates in ganA) analogous to pGP380; expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
- pGP1460: integration vector (integrates in ganA) analogous to pGP382; expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
- pGP1389: integrative plasmid for the fusion of a Strep-tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
Expression/ purification in E. coli
- pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
- pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
- pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
- pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein
Construction of (markerless) deletions
- pMAD: construction of markerless KOs in Gram-positive bacteria
A collection of biobricks for the work with B. subtilis
- the biobrick box PubMed
