ricT

ricT
168

subunit of the regulatory iron-sulfur containing RicA-RicF-RicT complex, required for RNase Y dependent maturation of polycistronic mRNAs, control of the phosphorelay, required for the achieving a sufficient level of Spo0A-P for sporulation initiation

Locus
BSU_00320
Molecular weight
31.07 kDa
Isoelectric point
4.84
Protein length
Gene length
Function
control of RNase Y activity, see Targets of the Y complex
Product
subunit of the regulatory iron-sulfur containing RicA-RicF-RicT complex
Essential
no
Synonyms
yaaT

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1774 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
41,657 → 42,484
Phenotypes of a mutant
block of sporulation at stage 0 PubMed
strongly reduced genetic competence, strongly reduced sporulation PubMed
defective in RNase Y-mediated maturation of polycistronic mRNAs, see Targets of the Y complex PubMed
defective in Swarming motility (prolonged period of immotility prior to Swarming) PubMed
The protein
Catalyzed reaction/ biological activity
the RicA-RicF-RicT complex acts as specificity factor for RNase Y-dependent processing of polycistronic mRNAs, see Targets of the Y complex PubMed
the RicA-RicF-RicT complex stimulates Spo0A phosphorylation in the phosphorelay PubMed
PSP1 C-terminal domain (aa 61-146) (according to UniProt)
two 4Fe-4S clusters (fully co-ordinates one cluster and contributes to the co-ordination of the second (with RicA and RicF)) PubMed
Structure
cytoplasm PubMed
cell membrane PubMed
cell periphery, depending on RNase Y PubMed
Expression and Regulation
Operons
Description
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xpaCyabC

2025-02-27 14:37:40

Jstuelk

188

adcd0181c3bf7f5a66d1644d176d0c60f2121a3a

87CEB0D67D1455F48A42F665A0B9A91B779AEC5D

Description
Regulation
expressed during growth and the transition phase, expression is erduced in stationary phase PubMed
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darAyabC

2025-02-20 12:59:23

ghost

175

62a125e6301e56c82f6a74dcb70741fe56737f41

DA0A2CE2876D38C223E4BA512D01D105B53BF136

Description
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tmkyabC

2025-02-26 07:59:20

Jstuelk

173

cae86a5ed186d19f5321f28f72d35514a5988afb

E9A27179465E2479F141CE804267881CAB860CC2

Additional information
600,000/ 11,500 molecules per cell during growth in LB/ minimal medium PubMed
Biological materials
Mutant
MGNA-B899 (ricT::erm), available at the NBRP B. subtilis, Japan
BKE00320 (ΔricT::erm  trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_GACACCAATTACATTGTACA,  downstream forward: _UP4_ACAGATTAACGAGGTGTGGA
BKK00320 (ΔricT::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_GACACCAATTACATTGTACA,  downstream forward: _UP4_ACAGATTAACGAGGTGTGGA
References
Reviews
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Original Publications
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EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4

Page visits: 6959

Time of last update: 2025-02-28 15:28:23

Author of last update: Jstuelk