Difference between revisions of "ParB"
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| − | * '''Description:''' chromosome positioning near the pole and transport through the polar septum / antagonist of [[Soj]]-dependent inhibition of [[sporulation]] initiation <br/><br/> | + | * '''Description:''' chromosome positioning near the pole and transport through the polar septum / antagonist of [[Soj]]-dependent inhibition of [[sporulation]] initiation, traps DNA loops, required for the recruitment of the [[SMC complex]] to the origin of replication <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 93: | Line 93: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''[[SubtInteract|Interactions]]:''' [[Spo0J]]-[[Soj]] {{PubMed|21235642}} | + | * '''[[SubtInteract|Interactions]]:''' |
| + | ** forms dimers {{PubMed|24829297}} | ||
| + | ** [[Spo0J]]-[[Soj]] {{PubMed|21235642}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| Line 127: | Line 129: | ||
** number of protein molecules per cell (minimal medium with glucose and ammonium): 341 {{PubMed|24696501}} | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 341 {{PubMed|24696501}} | ||
** number of protein molecules per cell (complex medium with amino acids, without glucose): 362 {{PubMed|24696501}} | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 362 {{PubMed|24696501}} | ||
| − | + | ** number of protein molecules per cell: 880 (equivalent to 440 dimers) {{PubMed|24829297}} | |
=Biological materials = | =Biological materials = | ||
| Line 154: | Line 156: | ||
<pubmed> 22934648 </pubmed> | <pubmed> 22934648 </pubmed> | ||
== Original publications == | == Original publications == | ||
| − | <pubmed>17462018 16677298 12562803, 10852876, 10482533, 9506522, 8071208, 17932079 19450517 21235642 9663676,11532141,19450516,15659156,18854156,9159397,8866474,9778525,16925562,1900505,9364919,9701805,12950914,,14651647, 19450517, 19450516, 14563866 23475963 21911367 24440393</pubmed> | + | <pubmed>17462018 16677298 12562803, 10852876, 10482533, 9506522, 8071208, 17932079 19450517 21235642 9663676,11532141,19450516,15659156,18854156,9159397,8866474,9778525,16925562,1900505,9364919,9701805,12950914,,14651647, 19450517, 19450516, 14563866 23475963 21911367 24440393 24829297 24696501</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:29, 18 May 2014
- Description: chromosome positioning near the pole and transport through the polar septum / antagonist of Soj-dependent inhibition of sporulation initiation, traps DNA loops, required for the recruitment of the SMC complex to the origin of replication
| Gene name | spo0J |
| Synonyms | parB, spo0JB |
| Essential | no |
| Product | site-specific DNA-binding protein |
| Function | chromosome positioning before asymmetric septation,
specifying its orientation, and imposing directionality on its subsequent transport through the septum; required for sporulation (antagonist of Soj-dependent inhibition of sporulation initiation) |
| Gene expression levels in SubtiExpress: spo0J | |
| Interactions involving this protein in SubtInteract: Spo0J | |
| MW, pI | 32 kDa, 8.417 |
| Gene length, protein length | 846 bp, 282 aa |
| Immediate neighbours | yyaC, soj |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA condensation/ segregation, sporulation/ other
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU40960
Phenotypes of a mutant
Database entries
- BsubCyc: BSU40960
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: parB family (according to Swiss-Prot)
- Paralogous protein(s): Noc
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- chromosome centromer PubMed
Database entries
- BsubCyc: BSU40960
- Structure:
- UniProt: P26497
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Heath Murray, Centre for Bacterial Cell Biology, Newcastle, UK homepage
Your additional remarks
References
Reviews
Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648]
[WorldCat.org]
[DOI]
(I p)
Original publications
