Difference between revisions of "ThiO"
| Line 124: | Line 124: | ||
** number of protein molecules per cell (minimal medium with glucose and ammonium): 556 {{PubMed|24696501}} | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 556 {{PubMed|24696501}} | ||
** number of protein molecules per cell (complex medium with amino acids, without glucose): 1224 {{PubMed|24696501}} | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1224 {{PubMed|24696501}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1342 {{PubMed|21395229}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1721 {{PubMed|21395229}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 593 {{PubMed|21395229}} | ||
=Biological materials = | =Biological materials = | ||
| − | |||
* '''Mutant:''' | * '''Mutant:''' | ||
Revision as of 14:07, 17 April 2014
- Description: FAD-dependent glycine oxidase
| Gene name | thiO |
| Synonyms | goxB, yjbR |
| Essential | no |
| Product | glycine oxidase |
| Function | biosynthesis of thiamine |
| Gene expression levels in SubtiExpress: thiO | |
| Metabolic function and regulation of this protein in SubtiPathways: thiO | |
| MW, pI | 40 kDa, 5.898 |
| Gene length, protein length | 1107 bp, 369 aa |
| Immediate neighbours | tenI, thiS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU11670
Phenotypes of a mutant
Database entries
- BsubCyc: BSU11670
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Glycine + H2O + O2 = glyoxylate + NH3 + H2O2 (according to Swiss-Prot)
- Protein family: DAMOX/DASOX family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: FAD PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions: the FAD-containing holoenzyme is a homotetramer PubMed
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU11670
- UniProt: O31616
- KEGG entry: [3]
- E.C. number: 1.4.3.19
Additional information
Expression and regulation
- Sigma factor:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 556 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 1224 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1342 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1721 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 593 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
