TrmFO

• Description: flavoprotein, tRNA:m(5)U-54 methyltransferase, glucose-inhibited division protein
  
  

Gene name	trmFO
Synonyms	ylyC, gid
Essential	no
Product	tRNA:m(5)U-54 methyltransferase
Function	tRNA modification
Gene expression levels in SubtiExpress: trmFO
MW, pI	47 kDa, 5.767
Gene length, protein length	1305 bp, 435 aa
Immediate neighbours	topA, codV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU16130

Database entries

• BsubCyc: BSU16130

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: catalyzes the folate-dependent C(5)-methylation of uridine at position 54 in the TpsiC loop of tRNA

• Protein family: TrmFO subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors: FAD, methylene-THF PubMed

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16130

• Structure:
  • 3G5S (TrmFO from Thermus thermophilus, 54% identity, 79% similarity) PubMed

• UniProt: P39815

• KEGG entry: [2]

• E.C. number: 2.1.1.74

Expression and regulation

• Operon:

• Expression browser: trmFO PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 458 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1069 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 237 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 237 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 439 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: