RasP

• Description: intramembrane protease, cleaves FtsL, RsiV and RsiW as well as signal peptides after release of the secreted proteins
  
  

Gene name	rasP
Synonyms	yluC
Essential	no
Product	intramembrane protease
Function	control of cell division, and SigV and SigW activity
Gene expression levels in SubtiExpress: rasP
Interactions involving this protein in SubtInteract: RasP
MW, pI	46 kDa, 5.14
Gene length, protein length	1266 bp, 422 aa
Immediate neighbours	ispC, proS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, proteolysis, sigma factors and their control, membrane proteins

The gene

Basic information

• Locus tag: BSU16560

Phenotypes of a mutant

• defects in competence development, protein secretion and membrane protein production PubMed
• mutants grow slower in liquid, are not competent, can’t activate SigW, have cell division defects, and decreased long term survival PubMed

Database entries

• BsubCyc: BSU16560

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • cleaves FtsL, RsiV and RsiW
  • cleaves signal peptides after release of the secreted proteins PubMed

• Protein family: peptidase M50B family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RasP-FtsL
  • RasP-RsiW
  • RasP-RsiV PubMed

• Localization: cell membrane PubMed

Database entries

• BsubCyc: BSU16560

• Structure:

• UniProt: O31754

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: rasP PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 24 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Thomas Wiegert, University of Bayreuth, Germany Homepage

References

Reviews

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)

Ross E Dalbey, Peng Wang, Jan Maarten van Dijl
Membrane proteases in the bacterial protein secretion and quality control pathway.
Microbiol Mol Biol Rev: 2012, 76(2);311-30
[PubMed:22688815] [WorldCat.org] [DOI] (I p)

Theresa D Ho, Craig D Ellermeier
Extra cytoplasmic function σ factor activation.
Curr Opin Microbiol: 2012, 15(2);182-8
[PubMed:22381678] [WorldCat.org] [DOI] (I p)

Gu Chen, Xu Zhang
New insights into S2P signaling cascades: regulation, variation, and conservation.
Protein Sci: 2010, 19(11);2015-30
[PubMed:20836086] [WorldCat.org] [DOI] (I p)

Michael S Wolfe
Intramembrane-cleaving proteases.
J Biol Chem: 2009, 284(21);13969-73
[PubMed:19189971] [WorldCat.org] [DOI] (P p)