Ndk

• Description: nucleoside diphosphate kinase
  
  

Gene name	ndk
Synonyms
Essential	no
Product	nucleoside diphosphate kinase
Function	maintainance of an equilibrium between the concentrations of different nucleoside triphosphates
Gene expression levels in SubtiExpress: ndk
Metabolic function and regulation of this protein in SubtiPathways: ndk
MW, pI	16 kDa, 5.568
Gene length, protein length	447 bp, 149 aa
Immediate neighbours	cheR, hepT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

nucleotide metabolism/ other, phosphoproteins

The gene

Basic information

• Locus tag: BSU22730

Database entries

• BsubCyc: BSU22730

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + nucleoside diphosphate = ADP + nucleoside triphosphate (according to Swiss-Prot)

• Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Thr-92 AND Ser-123 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU22730

• Structure: 1WKJ (from Thermus thermophilus, 59% identity, 82% similarity)

• UniProt: P31103

• KEGG entry: [2]

• E.C. number: 2.7.4.6

Expression and regulation

• Operon:

• Expression browser: ndk PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2721 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 39075 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)