Difference between revisions of "UppS"
| Line 55: | Line 55: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
* essential {{PubMed|22904278}} | * essential {{PubMed|22904278}} | ||
| + | * ''uppS'' is dispensable in wall-less L-forms {{PubMed|24704074}} | ||
* the ''[[uppS86]]'' (C257T) mutation provides resistance to rare earth metals such as scandium and to bacitracin {{PubMed|22904278}} | * the ''[[uppS86]]'' (C257T) mutation provides resistance to rare earth metals such as scandium and to bacitracin {{PubMed|22904278}} | ||
| Line 65: | Line 66: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
=The protein= | =The protein= | ||
| Line 81: | Line 81: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| Line 139: | Line 139: | ||
=References= | =References= | ||
| − | <pubmed> 22904278 23796923 15788389 23840410 </pubmed> | + | <pubmed> 22904278 23796923 15788389 23840410 24704074</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:43, 8 April 2014
- Description: undecaprenyl pyrophosphate synthetase, provides the precursor for the carrier lipid, undecaprenyl phosphate (C55-P), which is required for cell wall synthesis
| Gene name | uppS |
| Synonyms | yluA |
| Essential | yes PubMed |
| Product | undecaprenyl pyrophosphate synthetase |
| Function | cell wall biosynthesis |
| Gene expression levels in SubtiExpress: uppS | |
| Metabolic function and regulation of this protein in SubtiPathways: UppS | |
| MW, pI | 29 kDa, 5.408 |
| Gene length, protein length | 780 bp, 260 aa |
| Immediate neighbours | frr, cdsA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of lipids, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16530
Phenotypes of a mutant
- essential PubMed
- uppS is dispensable in wall-less L-forms PubMed
- the uppS86 (C257T) mutation provides resistance to rare earth metals such as scandium and to bacitracin PubMed
Database entries
- BsubCyc: BSU16530
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: catalyzes eight consecutive condensation reactions of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to form undecaprenyl pyrophosphate
- Protein family: UPP synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU16530
- UniProt: O31751
- KEGG entry: [2]
- E.C. number: 2.5.1.31
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yoshikazu Kawai, Romain Mercier, Jeff Errington
Bacterial cell morphogenesis does not require a preexisting template structure.
Curr Biol: 2014, 24(8);863-7
[PubMed:24704074]
[WorldCat.org]
[DOI]
(I p)
Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410]
[WorldCat.org]
[DOI]
(I e)
Yong Heon Lee, John D Helmann
Reducing the Level of Undecaprenyl Pyrophosphate Synthase Has Complex Effects on Susceptibility to Cell Wall Antibiotics.
Antimicrob Agents Chemother: 2013, 57(9);4267-4275
[PubMed:23796923]
[WorldCat.org]
[DOI]
(I p)
Takashi Inaoka, Kozo Ochi
Undecaprenyl pyrophosphate involvement in susceptibility of Bacillus subtilis to rare earth elements.
J Bacteriol: 2012, 194(20);5632-7
[PubMed:22904278]
[WorldCat.org]
[DOI]
(I p)
Rey-Ting Guo, Tzu-Ping Ko, Annie P-C Chen, Chih-Jung Kuo, Andrew H-J Wang, Po-Huang Liang
Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis.
J Biol Chem: 2005, 280(21);20762-74
[PubMed:15788389]
[WorldCat.org]
[DOI]
(P p)
