Difference between revisions of "GdpP"

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* inactivation of ''[[gdpP]]'' strongly restores beta-lactam resistance in a ''[[sigM]]'' mutant {{PubMed|22211522}}
 
* inactivation of ''[[gdpP]]'' strongly restores beta-lactam resistance in a ''[[sigM]]'' mutant {{PubMed|22211522}}
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40510&redirect=T BSU40510]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yybST-rplI.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yybST-rplI.html]
Line 93: Line 94:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40510&redirect=T BSU40510]
  
 
* '''Structure:'''
 
* '''Structure:'''

Revision as of 15:17, 2 April 2014

  • Description: cyclic di-AMP phosphodiesterase



Gene name gdpP
Synonyms yybT
Essential no
Product c-di-AMP phosphodiesterase
Function control of sporulation initiation, cell wall homeostasis
Gene expression levels in SubtiExpress: gdpP
MW, pI 74 kDa, 4.865
Gene length, protein length 1977 bp, 659 aa
Immediate neighbours rplI, yybS
Sequences Protein DNA DNA_with_flanks
Genetic context
YybT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GdpP expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU40510

Phenotypes of a mutant

  • inactivation of gdpP strongly restores beta-lactam resistance in a sigM mutant PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • cyclic dinucleotide phosphodiesterase, hydrolysis of di-cyclic AMP PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal domain that shares minimum sequence homology with Haem-binding Per-ARNT-Sim (PAS) domains (80 aa) PubMed
    • degenerate GGDEF domain (aa 173 - 301) PubMed
    • DHH/ DHHA1 phosphodiesterase domain PubMed
  • Modification:
  • Cofactor(s): b-type heme with 1:1 stoichiometry PubMed
  • Effectors of protein activity:
    • ppGpp inhibits cyclic dinucleotide phosphodiesterase activity PubMed
    • Heme binding suppresses phosphodiesterase activity PubMed

Database entries

  • Structure:
    • 2M1C (N-terminal PAS domain of GdpP from Geobacillus thermodenitrificans) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • there is an internal SigD-dependent antisense transcript (as-gdpP) PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

GdpP homologs in other organisms