SigV

• Description: RNA polymerase ECF-type sigma factor SigV
  
  

Gene name	sigV
Synonyms
Essential	no
Product	RNA polymerase ECF-type sigma factor SigV
Function	response to lysozyme
Gene expression levels in SubtiExpress: sigV
Interactions involving this protein in SubtInteract: SigV
MW, pI	19 kDa, 9.037
Gene length, protein length	498 bp, 166 aa
Immediate neighbours	yrhO, rsiV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigV regulon

The SigV regulon:

• sigV-rsiV-oatA-yrhK

The gene

Basic information

• Locus tag: BSU27120

Phenotypes of a mutant

• • increased sensitivity to lysozyme, this can be suppressed by overexpression of oatA PubMed

Database entries

• BsubCyc: BSU27120

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ECF subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • activity is controlled by the interaction with RsiV, proteolytic degradation of RsiV releases active SigV PubMed
• Interactions:
  • SigV-RsiV, this interaction inhibits the activity of SigV PubMed
  • SigV-(RpoB-RpoC)

• Localization:

Database entries

• BsubCyc: BSU27120

• Structure:

• UniProt: O05404

• KEGG entry: [3]

• E.C. number:

Additional information

• Expression of the SigV regulon in increased in ugtP mutants PubMed

Expression and regulation

• Operon: sigV-rsiV-oatA-yrhK PubMed

• Expression browser: sigV PubMed

• Sigma factor: SigV PubMed

• Regulation:
  • induced by lysozyme (SigV) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A907 ( sigV::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Thomas Wiegert, University of Bayreuth, Germany Homepage