Difference between revisions of "DarA"
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yaaQ_39871_40200_1 yaaQ] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yaaQ_39871_40200_1 yaaQ] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Mutant:''' | * '''Mutant:''' | ||
| − | ** GP1712 (''yaaQ''::''cat''), available in [[Jörg Stülke]]'s lab | + | ** GP1712 (''[[yaaQ]]''::''cat''), available in [[Jörg Stülke]]'s lab |
| − | ** GP1713 (''yaaQ- | + | ** GP1713 (''[[yaaQ]]-[[yaaR]]''::''cat''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | **pGP2601: | + | **pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Jörg Stülke]]'s lab |
**pGP2603: expression of ''yaaQ''-Strep by [[pGP382]] in ''B. subtilis'' suitable for [[SPINE]], available in [[Jörg Stülke]]'s lab | **pGP2603: expression of ''yaaQ''-Strep by [[pGP382]] in ''B. subtilis'' suitable for [[SPINE]], available in [[Jörg Stülke]]'s lab | ||
Revision as of 12:59, 14 March 2014
- Description: unknown
| Gene name | yaaQ |
| Synonyms | |
| Essential | |
| Product | unknown |
| Function | unknown |
| Gene expression levels in SubtiExpress: yaaQ | |
| MW, pI | 11 kDa, 6.088 |
| Gene length, protein length | 327 bp, 109 aa |
| Immediate neighbours | tmk, yaaR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00290
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Interactions:
- forms homo-trimers (see 3M05)
Database entries
- Structure:
- 3M05 (the protein from Pediococcus pentosaceus, 50% identity)
- UniProt: P37538
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1712 (yaaQ::cat), available in Jörg Stülke's lab
- GP1713 (yaaQ-yaaR::cat), available in Jörg Stülke's lab
- Expression vector:
- pGP2601: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
- pGP2603: expression of yaaQ-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
