Difference between revisions of "ScpA"

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* '''Description:''' DNA segregation and condensation protein <br/><br/>
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* '''Description:''' part of the [[condensin]] complex, chromosomal origin condensation and segregation <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || DNA segregation and condensation protein  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || DNA segregation and condensation protein  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || maintenance of chromosome structure
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|style="background:#ABCDEF;" align="center"|'''Function''' || segregation of replication origins
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23220 scpA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23220 scpA]
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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* ''[[scpA]]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth {{PubMed|24440399,24440393}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
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* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
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* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
 
* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
 +
** part of the [[condensin]] complex {{PubMed|24440399,24440393}}
 
** present as stable monomer {{PubMed|22385855}}
 
** present as stable monomer {{PubMed|22385855}}
 
** [[ScpA]]-[[Smc]] {{PubMed|23353789,22385855,12100548}}
 
** [[ScpA]]-[[Smc]] {{PubMed|23353789,22385855,12100548}}
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* '''[[Localization]]:'''  
 
* '''[[Localization]]:'''  
 
** nucleoid (Multiple) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
 
** nucleoid (Multiple) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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** the ([[Smc]])2-[[ScpA]]-[[ScpB]] complex forms two bipolar assemblies on the chromosome, one in each cell half {{PubMed|23475963}}
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** the [[condensin]] ([[Smc]])2-[[ScpA]]-[[ScpB]] complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on [[Spo0J]] {{PubMed|24440393,23475963}}
  
 
=== Database entries ===
 
=== Database entries ===
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== Original publications ==
 
== Original publications ==
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<pubmed>12100548,12421306,12065423,7934830, 16479537, 19450516 15009890 11948165 23353789 23475963 22385855</pubmed>
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<pubmed>12100548,12421306,12065423,7934830, 16479537, 19450516 15009890 11948165 23353789 23475963 22385855 24440399,24440393</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:28, 16 February 2014

  • Description: part of the condensin complex, chromosomal origin condensation and segregation

Gene name scpA
Synonyms ypuG
Essential yes PubMed
Product DNA segregation and condensation protein
Function segregation of replication origins
Gene expression levels in SubtiExpress: scpA
Interactions involving this protein in SubtInteract: ScpA
MW, pI 29 kDa, 4.788
Gene length, protein length 753 bp, 251 aa
Immediate neighbours scpB, ypuF
Sequences Protein DNA DNA_with_flanks
Genetic context
YpuG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ScpA expression.png















Categories containing this gene/protein

DNA condensation/ segregation, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU23220

Phenotypes of a mutant

  • essential PubMed
  • scpA mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: scpA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
    • 3ZGX (complex between the head domain of Smc (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of ScpA) PubMed
    • 4I98 (complex between ScpA (aa 1-160)-ScpB (aa 1-183) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)


Original publications

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