HelD

• Description: DNA 3'-5' helicase IV, stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation
  
  

Gene name	helD
Synonyms	yvgS
Essential	no
Product	DNA 3'-5' helicase IV
Function	transcription, DNA repair/ recombination
Gene expression levels in SubtiExpress: helD
MW, pI	89 kDa, 6.083
Gene length, protein length	2322 bp, 774 aa
Immediate neighbours	cysI, yvgT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, transcription

The gene

Basic information

• Locus tag: BSU33450

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UvrD subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • HelD interacts with the RNA polymerase core enzyme PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3DMN (C-terminal domain of the protein from Lactobacillus plantarum, 39% identity)

• UniProt: O32215

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: helD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A899 ( helD::erm), available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jana Wiedermannová, Petra Sudzinová, Tomaš Kovaľ, Alžbeta Rabatinová, Hana Šanderova, Olga Ramaniuk, Šimon Rittich, Jan Dohnálek, Zhihui Fu, Petr Halada, Peter Lewis, Libor Krásny
Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis.
Nucleic Acids Res: 2014, 42(8);5151-63
[PubMed:24520113] [WorldCat.org] [DOI] (I p)

Olivier Delumeau, François Lecointe, Jan Muntel, Alain Guillot, Eric Guédon, Véronique Monnet, Michael Hecker, Dörte Becher, Patrice Polard, Philippe Noirot
The dynamic protein partnership of RNA polymerase in Bacillus subtilis.
Proteomics: 2011, 11(15);2992-3001
[PubMed:21710567] [WorldCat.org] [DOI] (I p)

B Carrasco, S Fernández, M A Petit, J C Alonso
Genetic recombination in Bacillus subtilis 168: effect of DeltahelD on DNA repair and homologous recombination.
J Bacteriol: 2001, 183(19);5772-7
[PubMed:11544244] [WorldCat.org] [DOI] (P p)