Difference between revisions of "WalJ"
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 804 bp, 268 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 804 bp, 268 aa | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[htrC]]'', ''[[yycI]]'' |
|- | |- | ||
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40370 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40370 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40370 DNA_with_flanks] | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40370 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40370 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40370 DNA_with_flanks] | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''[[SubtInteract|Interactions]]:''' | + | * '''[[SubtInteract|Interactions]]:''' |
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yycJ_4148851_4149645_-1 walJ] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yycJ_4148851_4149645_-1 walJ] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' expressed during vegetative growth, repressed during stationary phase [http://www.ncbi.nlm.nih.gov/sites/entrez/9829949 PubMed] | * '''Regulation:''' expressed during vegetative growth, repressed during stationary phase [http://www.ncbi.nlm.nih.gov/sites/entrez/9829949 PubMed] | ||
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=References= | =References= | ||
| − | + | <pubmed>9829949 21169496,21205011</pubmed> | |
| − | <pubmed>9829949</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 17:12, 15 January 2014
- Description: coordination of cell division with DNA replication
| Gene name | walJ |
| Synonyms | yycJ |
| Essential | no |
| Product | unknown |
| Function | coordination of cell division with DNA replication |
| Gene expression levels in SubtiExpress: walJ | |
| MW, pI | 29 kDa, 5.503 |
| Gene length, protein length | 804 bp, 268 aa |
| Immediate neighbours | htrC, yycI |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell division, cell wall synthesis
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU40370
Phenotypes of a mutant
- the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited PubMed
- increased sensitivity to a narrow spectrum of cephalosporin antibiotics PubMed
- disruption of the corresponding gene in B. anthracis results in a mutator phenotype PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of cell division with DNA replication PubMed
- Protein family: predicted member of the metallo-β-lactamase superfamily
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: C0SP91
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: expressed during vegetative growth, repressed during stationary phase PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Hanjing Yang, Cameron Sikavi, Katherine Tran, Shauna M McGillivray, Victor Nizet, Madeline Yung, Aileen Chang, Jeffrey H Miller
Papillation in Bacillus anthracis colonies: a tool for finding new mutators.
Mol Microbiol: 2011, 79(5);1276-93
[PubMed:21205011]
[WorldCat.org]
[DOI]
(I p)
Steven J Biller, Kyle J Wayne, Malcolm E Winkler, William F Burkholder
The putative hydrolase YycJ (WalJ) affects the coordination of cell division with DNA replication in Bacillus subtilis and may play a conserved role in cell wall metabolism.
J Bacteriol: 2011, 193(4);896-908
[PubMed:21169496]
[WorldCat.org]
[DOI]
(I p)
C Fabret, J A Hoch
A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy.
J Bacteriol: 1998, 180(23);6375-83
[PubMed:9829949]
[WorldCat.org]
[DOI]
(P p)
