FliW

• Description: checkpoint protein for hag expression, CsrA anatagonist
  
  

Gene name	fliW
Synonyms	yviF
Essential	no
Product	CsrA anatagonist
Function	control of CsrA activity
Gene expression levels in SubtiExpress: fliW
Interactions involving this protein in SubtInteract: FliW
MW, pI	16 kDa, 4.338
Gene length, protein length	429 bp, 143 aa
Immediate neighbours	csrA, yviE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis

The gene

Basic information

• Locus tag: BSU35380

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • FliW binds CsrA when Hag is secreted after hook completion (and FliW released from the Hag-FliW complex) to allow translation of the hag mRNA PubMed

• Protein family: fliW family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • FliW-Hag PubMed
  • FliW-CsrA PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P96503

• KEGG entry: [2]

• E.C. number:

Additional information

• CsrA and FliW form a conserved module in many bacteria PubMed

Expression and regulation

• Operon:

• Expression browser: fliW PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Additional Reviews: PubMed

Original Publications

Sampriti Mukherjee, Paul Babitzke, Daniel B Kearns
FliW and FliS function independently to control cytoplasmic flagellin levels in Bacillus subtilis.
J Bacteriol: 2013, 195(2);297-306
[PubMed:23144244] [WorldCat.org] [DOI] (I p)

Sampriti Mukherjee, Helen Yakhnin, Dave Kysela, Josh Sokoloski, Paul Babitzke, Daniel B Kearns
CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis.
Mol Microbiol: 2011, 82(2);447-61
[PubMed:21895793] [WorldCat.org] [DOI] (I p)

Björn Titz, Seesandra V Rajagopala, Claudia Ester, Roman Häuser, Peter Uetz
Novel conserved assembly factor of the bacterial flagellum.
J Bacteriol: 2006, 188(21);7700-6
[PubMed:16936039] [WorldCat.org] [DOI] (P p)