AcoB

• Description: acetoin dehydrogenase E1 component (TPP-dependent beta subunit)
  
  

Gene name	acoB
Synonyms	yfjJ
Essential	no
Product	acetoin dehydrogenase E1 component (TPP-dependent beta subunit)
Function	acetoin utilization
Gene expression levels in SubtiExpress: acoB
Interactions involving this protein in SubtInteract: AcoB
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	36 kDa, 4.396
Gene length, protein length	1026 bp, 342 aa
Immediate neighbours	acoA, acoC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AcoR regulon, CcpA regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU08070

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): PdhB, BkdAB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (AcoA-AcoB)-AcoC-AcoL

• Localization: Membrane-proximal (Spotty) PubMed

Database entries

• Structure:

• UniProt: O34591

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: acoA-acoB-acoC-acoL PubMed

• Expression browser: acoB PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by acetoin (AcoR) PubMed
  • induced under anaerobic conditions (Fnr) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AcoR: transcription activation (interaction with SigL-containing RNA polymerase) PubMed
  • Fnr: indirect positive regulation via NarG-NarH-NarJ-NarI PubMed

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage